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. Author manuscript; available in PMC: 2024 Aug 19.
Published in final edited form as: Nature. 2023 Jul 26;621(7977):154–161. doi: 10.1038/s41586-023-06456-z

Fig. 2 |. RNA/DNA-bound tetramer.

Fig. 2 |

a, b, Cryo-EM density (a) and ribbon diagrams (b) of tetrameric MapSPARTA in complex with guide RNA (red)-target DNA (blue) duplex. The four protomers of MapSPARTA are colored in green, blue, yellow, and pink, respectively. The four protomers in adopt two different conformations, indicated as State I and State II.

c, d, Ribbon diagrams of MapSPARTA in State I (c) and State II (d). TIR is tightly associated with Ago in State I and loosely in State II.

e, Overlaid structures of MapSPARTA in State I and State II, revealing a dramatic 180º rotation in the TIR domain.

f, Ribbon diagram of MID-MID interaction. The region of interest in shown as a red oval.

g, Electrostatically complementary interface between two MID domains.

h, Detailed MID-MID interactions with interfacial residues highlighted in sticks.