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. Author manuscript; available in PMC: 2024 Aug 19.
Published in final edited form as: Nature. 2023 Jul 26;621(7977):154–161. doi: 10.1038/s41586-023-06456-z

Extended Data Fig. 3 |. Interfaces between TIR-APAZ and pAgo.

Extended Data Fig. 3 |

a, Interfaces between TIR-APAZ and pAgo with pAgo showing in electrostatic surface and TIR-APAZ in ribbon diagram.

b, Surface areas of different interfaces illustrated in panel a.

c, Interfaces between the APAZ domain and the PIWI domain. Key residues on the interfaces were highlighted in sticks.

d, Interfaces between the APAZ domain and the MID domain. Key residues on the interfaces were highlighted in sticks.

e, Detailed interactions between the TIR domain and the MID domain. Key residues on the interfaces were highlighted in sticks.

f, Representative kinetics data of NAD+ hydrolysis by wildtype and CTM-truncated MapSPARTA.

g, Quantitation of the catalytic activities of wildtype and CTM-truncated MapSPARTA. Data are mean ± SD from 3 or more replicates as indicated (Wild type, n=6; CTM-truncated SPARTA, n=3).