Figure 1.

Summary of differentiation protocol

hiPSCs are passaged to form small colonies, the next day at day 0, treatment with the first growth factor combination is induced from days 0–3 to form mesoderm progenitor cells. From days 3–7, new growth factors are introduced to form hematopoietic progenitor cells (HPCs). From days 7–10 new growth factors are introduced to enhance HPC proliferation. From days 10–14 new growth factors are introduced to promote development of myeloid progenitor cells that float on top of the monolayer culture. Floating myeloid cells are replated on low-attached plates and treated with growth factors from day 14–21 to enhance production of monocytes and other mature myeloid lineage cells. Monocytes can be further cultured to resting M0 macrophages from days 21–28 and these cells can be polarized to classical M1 and M2 states by additional growth factor treatment for 24 h. Created with license from BioRender.