REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
PE-conjugated mouse monoclonal antibody anti-human CD34 (clone: 561, 1:20)	BioLegend	Cat# 343606; RRID: AB_1732033
APC-conjugated mouse monoclonal antibody anti-human CD45 (clone: H130, 1:20)	BioLegend	Cat# 304012; RRID: AB_314399
FITC-conjugated mouse monoclonal antibody anti-human CD14 (clone: GCD14, 1:20)	BioLegend	Cat# 325604; RRID: AB_830677
PE Cy5-conjugated mouse monoclonal antibody anti-human CD11b (clone: IRCF44, 1:20)	BioLegend	Cat# 301308; RRID: AB_314160
PE-conjugated mouse monoclonal antibody anti-human CD209 (clone: DCN46, 1:20)	BD	Cat# 551265; RRID: AB_394123
FITC-conjugated mouse monoclonal antibody anti-human CD80 (clone: L307-4, 1:20)	BD	Cat# 557226; RRID: AB_396605
Mouse monoclonal antibody anti-human CD68 (clone: KP1, 1:200)	Dako	Cat# M0814; RRID: AB_2314148
AF568-conjugated goat anti-mouse antibody (1:1,000)	Invitrogen	Cat# A-11004; RRID: AB_2534072
Chemicals, peptides, and recombinant proteins
Matrigel hESC-qualified matrix	Corning	354277
DMEM/F-12	Thermo Fisher Scientific	11320033
StemFlex medium	Thermo Fisher Scientific	A3349401
STEMdiff APEL 2 medium	STEMCELL Technologies	05275
Protein-free hybridoma mixture II (PFHMII)	Invitrogen	12040-077
Penicillin/streptomycin (50 U Pen G/50 mg streptomycin sulfate)	Invitrogen	15140122
ReLeSR (enzyme-free dissociation reagent)	STEMCELL Technologies	100-0483
Y-27632 dihydrochloride (Rock inhibitor)	Tocris	1254
Recombinant human (rh) vascular endothelial growth factor (rhVEGF)	PeproTech	100-20
rh bone morphogenetic protein 4 (rhBMP4)	R&D Systems	314-BP-050
rh basic fibroblast growth factor (rhbFGF)	PeproTech	100-18B
rh stem cell factor (rhSCF)	STEMCELL Technologies	02830
rh Flt-3 ligand (rhFlt-3L)	STEMCELL Technologies	02941
rh thrombopoietin (rhTPO)	STEMCELL Technologies	02720
rh granulocyte-macrophage colony-stimulating factor (rhGM-CSF)	PeproTech	300-03
rh macrophage colony-stimulating factor (rhM-CSF)	PeproTech	300-25
Lipopolysaccharides (LPS) purified from Escherichia coli (E. coli) 055:B5	Sigma-Aldrich	L2880-10MG
rh interferon-gamma (rhIFN-γ)	PeproTech	300-02
rh interleukin-4 (rhIL-4)	PeproTech	200-04
RPMI 1640 medium without L-glutamine with phenol red	Euroclone	ECB9006L
L-glutamine 200 mM	PAN-Biotech	P04-80100
PENICILLIN-STREPTOMICIN 100X	Euroclone	ECB3001D
Fetal bovine serum (FBS)	Sigma-Aldrich	F0926
Accutase	Merck-Millipore	SCR005
Low endotoxin bovine serum albumin (BSA)	Sigma-Aldrich	A9418-5G
Dulbecco’s phosphate-buffered saline (PBS) without calcium (Ca++) and magnesium (Mg++) (DPBS −/−)	Sigma-Aldrich	D8537
H2O
Glass coverslip (round, 14 mm)	Thermo Fisher Scientific	10337423
Deionized H2O
Hydrochloric acid (HCl)	Sigma-Aldrich	320331-500ML
Ethyl alcohol (EtOH)	Sigma-Aldrich	792802
Dulbecco’s phosphate-buffered saline (DPBS) containing calcium (Ca++) and magnesium (Mg++) (DPBS +/+)	Sigma-Aldrich	D1283
Paraformaldehyde solution (PFA) 4% in PBS	Santa Cruz	30525-89-4
Tween 20	Sigma-Aldrich	P1379-100ML
Triton X-100	Sigma-Aldrich	X100-100ML
Normal goat serum (NGS)	Abcam	ab7481
DAPI (4′,6-diamidino-2-phenylindole, dihydrochloride)	Invitrogen	D1306
ProLong Gold antifade reagent	Invitrogen	P36934
EDTA solution pH 8.0 (0.5 M)	Euroclone	APA48920500
Human TruStain FcX (Fc receptor blocking solution)	BioLegend	422301
Zombie Aqua fixable viability kit	BioLegend	423102
BD CompBeads anti-mouse Ig, κ/negative control compensation particles set	BD	552843
HEPES	Sigma-Aldrich	H4034-25G
Hanks’ balanced salt solution (HBSS) without calcium (Ca++) and magnesium (Mg++) (HBSS −/−)	Thermo Fisher Scientific	14170120
Critical commercial assays
pHrodo Green Zymosan BioParticles conjugate for phagocytosis	Invitrogen	P35365
Experimental models: Cell lines
hiPSC NC6 (line 1 in figures, protocol 10-H-0126, control)	In-house generated	N/A
hiPSC NC8 (line 2 in figures, protocol 10-H-0126, control)	In-house generated	N/A
hiPSC ND7C (line 3 in figures, protocol 10-H-0126, disease)	In-house generated	N/A
hiPSC NC10 (protocol 10-H-0126, control)	In-house generated	N/A
hiPSC NC4 (protocol 10-H-0126, control)	In-house generated	N/A
hiPSC ND7B (protocol 10-H-0126, disease)	In-house generated	N/A
hiPSC ND7D (protocol 10-H-0126, disease)	In-house generated	N/A
Human primary monocytes	In-house generated	N/A
Software and algorithms
FlowJo X 10.0.7r2 software	BD	https://www.bdbiosciences.com/en-us/products/software/flowjo-v10-software; RRID: SCR_008520
Other
Non-pyrogenic Costar clear TC-treated multiple well tissue culture plates	Corning	3506 (6-well), 3512 (12-well), 3527 (24-well)
Corning 100 mm (10 cm) TC-treated culture dish	Corning	430167
0.22 mm filter units-50 mL	Sigma	SCGP00525
ZEISS Primovert inverted cell culture dissection microscope	ZEISS	N/A
TC20 automated cell counter	Bio-Rad	1450102
Cell counting slides for TC20	Bio-Rad	1450015
Olympus Fluoview FV1000 (laser scanning confocal microscopy)	Olympus	N/A
MACSQuant Analyzer 10 flow cytometer	Miltenyi Biotec	N/A
LSRFortessa flow cytometer	Beckton Dickinson	N/A
FACSAria	Beckton Dickinson	N/A