Basal macrophage M0 medium (days 21–28)

Reagent	Final concentration	Amount
L-Glutamine	N/A	500 μL
Penicillin-streptomycin	N/A	500 μL
M-CSF	100 ng/mL	50 μL
Basal late stage differentiation medium	N/A	50 mL
Total	N/A	51 mL

Note: Sterilize media using a 0.22 μm membrane filter and store at 4°C for maximum 7 days. Pre-warm the culture medium for 15 min at room temperature before use.