Reagent | Final concentration | Amount |
---|---|---|
Matrigel hESC-qualified matrix stock | N/A | 500 μL |
DMEM/F12 | N/A | 49.5 mL |
Total | N/A | 50 mL |
Note: Store at 4°C for maximum 4 weeks. Pre-warm the culture medium for 15 min at room temperature before use.
Reagent | Final concentration | Amount |
---|---|---|
Matrigel hESC-qualified matrix stock | N/A | 500 μL |
DMEM/F12 | N/A | 49.5 mL |
Total | N/A | 50 mL |
Note: Store at 4°C for maximum 4 weeks. Pre-warm the culture medium for 15 min at room temperature before use.