hiPSC culture StemFlex medium

Reagent	Final concentration	Amount
StemFlex Basal Medium	N/A	450 mL
StemFlex Supplement	10%	50 mL
Rock inhibitor, 10 mM (the day of passage only)	10 μM	10 μL in 10 mL medium
Total	N/A	500 mL

Note: Sterilize media using a 0.22 μm membrane filter and store at 4°C for maximum 14 days. Pre-warm the culture medium for 15 min at room temperature before use.