Basal early-stage differentiation medium

Reagent	Final concentration	Amount
STEMdiff APEL 2 medium	N/A	47.5 mL
Protein free hybridoma medium (PFHM-II)	5%	2.5 mL
Total	N/A	50 mL

Note: Store at 4°C for maximum 14 days. Pre-warm the culture medium for 15 min at room temperature before use.