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Mesoderm induction medium (days 0–3)
| Reagent | Final concentration | Amount |
|---|---|---|
| BMP4 | 10 ng/mL | 5 μL |
| VEGF | 10 ng/mL | 5 μL |
| bFGF | 10 ng/mL | 5 μL |
| Basal early-stage differentiation medium | N/A | 50 mL |
| Total | N/A | 50 mL |
Note: Sterilize media using a 0.22 μm membrane filter and store at 4°C for maximum 7 days. Pre-warm the culture medium for 15 min at room temperature before use.
