HPC expansion medium (days 7–10)

Reagent	Final concentration	Amount
BMP4	10 ng/mL	5 μL
VEGF	10 ng/mL	5 μL
bFGF	10 ng/mL	5 μL
SCF	50 ng/mL	25 μL
TPO	50 ng/mL	25 μL
FLT-3L	50 ng/mL	25 μL
GM-CSF	100 ng/mL	50 μL
Basal early-stage differentiation medium	N/A	50 mL
Total	N/A	50 mL

Note: Sterilize media using a 0.22 μm membrane filter and store at 4°C for maximum 7 days. Pre-warm the culture medium for 15 min at room temperature before use.