Basal late-stage differentiation medium

Reagent	Final concentration	Amount
Fetal bovine serum (FBS)	10%	5 mL
RPMI 1640 medium	N/A	45 mL
Total	N/A	50 mL

Note: Sterilize media using a 0.22 μm membrane filter and store at 4°C for maximum 7 days. Pre-warm the culture medium for 15 min at room temperature before use.