| Antibodies |
| Anti-HA (12CA5) |
Roche |
RRID# AB_514505 |
| Anti-HA (ChIP) |
abcam |
ab9110 |
| Chemicals, peptides, and recombinant proteins |
| Formaldehyde Solution |
Sigma-Aldrich |
Cat# 252549 |
| Proteinase K |
ThermoFisher |
Cat# EO0491 |
| GelRed Nucleic Acid Gel Stain |
Biotium |
Cat# 41103 |
| 5-Fluoroorotic Acid |
Zymo Research |
Cat# F9001 |
| Micrococcal nuclease |
Worthington Biochemical |
Cat# LS004798
|
| Propidium iodide solution |
Sigma-Aldrich |
Cat# P4864 |
| Triton X-100 |
Sigma-Aldrich |
Cat# T9284 |
| NP-40 Alternative |
Sigma-Aldrich |
Cat# 492016 |
| Sodium Deoxycholate |
Sigma-Aldrich |
Cat# S1827 |
| cOmplete Protease Inhibitors Cocktail |
Sigma-Aldrich |
Cat# 11836145001 |
| Bovine serum albumin |
Sigma-Aldrich |
Cat# 9048–46-8 |
| Sodium azide |
VWR |
Cat# 97064–646 |
| RNase A |
ThermoFisher |
Cat# EN0531 |
| Critical commercial assays |
| Dynabeads Protein G |
Invitrogen |
Cat# 10004D |
| SYBR Green Master Mix |
ThermoFisher |
Cat# A25742 |
| Deposited data |
| Raw data presented in this manuscript |
This study |
https://www.stowers.org/research/publications/libpb-2437
|
| Original Code used in the manuscript |
This study |
https://github.com/jmvarberg/Kunchala_et_al_2024
|
| Experimental models: Organisms/strains |
| All Saccharomyces cerevisiae strains used in this study are listed in Table S1
|
Lab stock and this study |
N/A |
| Oligonucleotides |
| Oligonucleotides used in this study are listed in Table S3
|
|
N/A |
| Recombinant DNA |
| Yeast MOBY collection |
Ho et al.39
|
DOI:10.1038/nbt.1534 |
| Yeast HIP ORF collection |
Hu et al.15
|
DOI:10.1101/gr.6037607 |
| Software and algorithms |
| Fiji (Image J) V2.3.1/1.53f |
https://imageJ.net/
|
RRID:SCR_002285 |
| FlowJo v9 |
FlowJo |
RRID# SCR_008520 |
| Prism V9.3.1 |
GraphPad |
RRID# SCR_002798 |
| R v 4.2.1 |
R core team |
RRID# SCR_001905 |
