Key resources table.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (Mus musculus) |
Neurog1:CreERT2 | The Jackson Laboratory | RRID:IMSR_JAX:008529 | A BAC transgenic mouse strain |
Strain, strain background (Mus musculus) |
Ai14 (tdTomato) |
The Jackson Laboratory | RRID:IMSR_JAX:007914 | A knock-in mouse strain |
Strain, strain background (Mus musculus) |
Ntn1β/β | Serafini et al., 1996 | RRID:MMRRC_030660-UCD | A gene trap mouse strain |
Strain, strain background (Mus musculus) |
Slit1;Slit2 | Plump et al., 2002 | RRID:MMRRC_030404-MU | A knock-out mouse strain |
Strain, strain background (Mus musculus) |
Dcc | Fazeli et al., 1997 | RRID:MMRRC_030626-MU | A knock-out mouse strain |
Strain, strain background (Mus musculus) |
Robo1;Robo2 | Ma and Tessier-Lavigne, 2007 | RRID:MMRRC_030747-MU | A knock-out mouse strain |
Antibody | anti-neurofilament (Mouse monoclonal) | DSHB | Cat# 2H3, RRID:AB_531793 |
IF(1:200) |
Antibody | anti-RFP (Rabbit polyclonal) | Rockland | Cat# 600-401-379, RRID:AB_2209751 | IF(1:500) |
Antibody | Cy5-AffiniPure Donkey Anti-Mouse IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 715-175-150 RRID:AB_2340819 | IF(1:500) |
Antibody | Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L) | Jackson ImmunoResearch Labs | Cat# 711-165-152, RRID:AB_2307443 | (IF1:500) |
Other | Dil | Invitrogen | Cat# D282 | 0.5% in ethanol |
Software, algorithm | PRISM 10 | GraphPad | RRID: SCR_002798 | https://www.graphpad.com/scientific-software/prism/ |
Software, algorithm | Leica Application Suite X | Leica Microsystems | RRID:SCR_013673 | https://www.leica-microsystems.com/products/microscope-software/details/product/leica-las-x-ls/ |
Software, algorithim | FIJI | Schindelin et al., 2012 | RRID:SCR_002285 | https://fiji.sc |