Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | C57BL/6 J | Jackson Laboratory | Stock No. 000664, RRID:IMSR_JAX:000664 | |
| Strain, strain background (Mus musculus) | CD45.1 | Jackson Laboratory | Stock No. 002014, RRID:IMSR_JAX:002014 | |
| Strain, strain background (Mus musculus) |
Cyria-/- (C57BL/6 J background) |
Generation of knockout mice in this paper | ||
| Strain, strain background (Mus musculus) |
Cyrib-/- (C57BL/6 J background) |
Generation of knockout mice in this paper | ||
| Antibody | Anti-human Fam49a (Rabbit polyclonal) |
Millipore | Cat# SAB1103179 RRID:AB_10900801 |
WB (1:1000) |
| Antibody | Anti-Fam49b (Mouse monoclonal, Clone D-8) | Santa Cruz | Cat# SC-390478 | WB (1:2000) |
| Antibody | Anti-GAPDH (Rabbit polyclonal) | Abcam | Cat# ab9485, RRID:AB_307275 | WB (1:2000) |
| Antibody | anti-PLCγ1 (Rabbit monoclonal, Clone D9H10) | Cell Signaling Technology | Cat# 5690 RRID:AB_10691383 |
WB (1:1000) |
| Antibody | anti-p-PLCγ1 (Rabbit polyclonal) | Cell Signaling Technology | Cat# 2821 RRID:AB_330855 |
WB (1:1000) |
| Antibody | anti-ZAP-70 (Rabbit monoclonal, Clone 99F2) | Cell Signaling Technology | Cat# 2705 RRID:AB_2273231 |
WB (1:1000) |
| Antibody | anti-p-ZAP-70 (Rabbit monoclonal, Clone 65E4) | Cell Signaling Technology | Cat# 2717 RRID:AB_2218658 |
WB (1:1000) |
| Antibody | anti-p44/42 MAPK (Erk1/2) (Rabbit polyclonal) | Cell Signaling Technology | Cat# 9102 RRID:AB_330744 |
WB (1:1000) |
| Antibody | anti-p-p44/42 MAPK (Erk1/2) (Rabbit monoclonal, Clone 197G2) | Cell Signaling Technology | Cat# 4377 RRID:AB_331775 |
WB (1:1000) |
| Antibody | anti-p-LAT (Rabbit monoclonal, Clone E3S5L) | Cell Signaling Technology | Cat# 20172 RRID:AB_3073971 |
WB (1:1000) |
| Antibody | anti-LAT (Rabbit monoclonal, Clone E3U6J) | Cell Signaling Technology | Cat# 45533 | WB (1:1000) |
| Antibody | anti-BIM (Rabbit monoclonal, Clone C34C5) | Cell Signaling Technology | Cat# 2933 RRID:AB_1030947 |
WB (1:1000) |
| Antibody | anti-Bcl-2 (Rabbit monoclonal, Clone D17C4) | Cell Signaling Technology | Cat# 3498 RRID:AB_1903907 |
WB (1:1000) |
| Antibody | anti-p-PAK1 (Ser144)/ PAK2 (Ser141) (Rabbit polyclonal) | Cell Signaling Technology | Cat# 2606 RRID:AB_2299279 |
WB (1:1000) |
| Antibody | anti-p-PAK1 (Ser199/204)/ PAK2 (Ser192/197) (Rabbit polyclonal) | Cell Signaling Technology | Cat# 2605 RRID:AB_2160222 |
WB (1:1000) |
| Antibody | anti-p-PAK1 (Thr423)/ PAK2 (Thr402) (Rabbit polyclonal) | Cell Signaling Technology | Cat# 2601 RRID:AB_330220 |
WB (1:1000) |
| Antibody | anti-PAK1 (Rabbit polyclonal) | Cell Signaling Technology | Cat# 2602 RRID:AB_330222 |
WB (1:1000) |
| Antibody | anti-PAK2 (Rabbit monoclonal, Clone C17A10) | Cell Signaling Technology | Cat# 2615 RRID:AB_2267950 |
WB (1:1000) |
| Antibody | anti-PAK3 (Rabbit polyclonal) | Cell Signaling Technology | Cat# 2609 RRID:AB_2225298 |
WB (1:1000) |
| Antibody | anti-PAK1/2/3 (Rabbit polyclonal) | Cell Signaling Technology | Cat# 2604 RRID:AB_2160225 |
WB (1:1000) |
| Antibody | Anti-mouse IgG (Dunkey polyclonal) | Licor | Cat# 926–68072, RRID:AB_10953628 | WB (1:10000) |
| Antibody | Anti-Rabbit IgG (Goat polyclonal) | Licor | Cat# 926–32211, RRID:AB_621843 | WB (1:1000) |
| Antibody | Anti-CD3ε-FITC (Rat monoclonal, Clone 17A2) | BioLegend | Cat# 100204, RRID:AB_312661 | FC (1:200) |
| Antibody | Anti-CD4-APC (Rat monoclonal, Clone RM4-5) | BioLegend | Cat# 100516, RRID:AB_312719 | FC (1:200) |
| Antibody | Anti-CD5-APC-Cy7 (Rat monoclonal, Clone 53–7.3) | BioLegend | Cat# 100649, RRID:AB_2860587 | FC (1:200) |
| Antibody | Anti-CD8α-BV421 (Rat monoclonal, Clone 53–6.7) | BioLegend | Cat# 100738, RRID:AB_11204079 | FC (1:200) |
| Antibody | Anti-CD8β-PE-Cy7 (Rat monoclonal, Clone 53–5.8) | BioLegend | Cat# 140416, RRID:AB_2564385 | FC (1:200) |
| Antibody | Anti-CD11b-PerCP-Cy5.5 (Rat monoclonal, Clone M1/70) | BioLegend | Cat# 101228, RRID:AB_893232 | FC (1:200) |
| Antibody | Anti-CD19-PerCP-Cy5.5 (Rat monoclonal, Clone 6D5) | BioLegend | Cat# 115534, RRID:AB_2072925 | FC (1:200) |
| Antibody | Anti-CD24-FITC (Rat monoclonal, Clone M1/69) | BioLegend | Cat# 101806, RRID:AB_312839 | FC (1:200) |
| Antibody | Anti-CD25-APC (Rat monoclonal, Clone PC61) | BioLegend | Cat# 102012, RRID:AB_312861 | FC (1:200) |
| Antibody | Anti-CD44-Pacific Blue (Rat monoclonal, Clone IM7) | BioLegend | Cat# 103020, RRID:AB_493683 | FC (1:200) |
| Antibody | Anti-CD45-Pacific Blue (Rat monoclonal, Clone 30-F11) | BioLegend | Cat# 103126, RRID:AB_493535 | FC (1:200) |
| Antibody | Anti-CD45.1-PE (Mouse monoclonal, Clone A20) | BioLegend | Cat# 110708, RRID:AB_313497 | FC (1:200) |
| Antibody | Anti-CD45.2-FITC (Mouse monoclonal, Clone 104) | BioLegend | Cat# 109806, RRID:AB_313443 | FC (1:200) |
| Antibody | Anti-CD45R/B220-PerCP-Cy5.5 (Rat monoclonal, Clone RA3-6B2) | BioLegend | Cat# 103236, RRID:AB_893354 | FC(1:400) |
| Antibody | Anti-CD62L-APC (Rat monoclonal, Clone MEL-14) | BioLegend | Cat# 104412, RRID:AB_313099 | FC (1:200) |
| Antibody | Anti-CD69-PE (Armenian Hamster monoclonal, Clone H1.2F3) | BioLegend | BioLegend Cat# 104507, RRID:AB_313110 | FC (1:200) |
| Antibody | Anti-CD103-Alexa Fluor 700 (Armenian Hamster monoclonal, Clone 2E7) | BioLegend | BioLegend Cat# 121442, RRID:AB_2813993 | FC (1:200) |
| Antibody | Anti-CD197/CCR7-BV421 (Rat monoclonal, Clone 4B12) | BioLegend | Cat# 120120, RRID:AB_2561446 | FC (1:50) |
| Antibody | Anti-Ly6G-PerCP-Cy5.5 (Rat monoclonal, Clone 1A8) | BioLegend | Cat# 127616, RRID:AB_1877271 | FC (1:200) |
| Antibody | Anti-Ly6C-PerCP-Cy5.5 (Rat monoclonal, Clone HK1.4) | BioLegend | Cat# 128012, RRID:AB_1659241 | FC (1:200) |
| Antibody | Anti-NK1.1-PerCP-Cy5.5 (Mouse monoclonal, Clone PK136) | BioLegend | Cat# 108728, RRID:AB_2132705 | FC (1:200) |
| Antibody | Anti-TCRβ-PE/Cy7 (Armenian Hamster monoclonal, Clone H57-597) | BioLegand | Cat# 109222, RRID:AB_893625 | FC (1:200) |
| Antibody | Anti-TCRγδ-PE (Armenian Hamster monoclonal, Clone GL3) | BioLegend | Cat# 118108, RRID:AB_313832 | FC (1:200) |
| Antibody | Anti-CD16/CD32 (Rat monoclonal, Clone 93) | Thermo Fisher Scientific | Cat# 14-0161-85, RRID:AB_467134 | FC (1:200) |
| Antibody | Anti-Cleaved Caspase 3-PE (Rabbit monoclonal, Clone D3E9) | Cell Signaling Technology | Cat# 12768, RRID:AB_2798021 | FC (1:50) |
| Antibody | Anti-Foxp3-PE (Rab monoclonal, Clone FJK16s) | eBioscience | Cat# 12-5773-82, RRID:AB_465936 | FC (1:200) |
| Antibody | Anti-TCRVα2-FITC (Rat monoclonal, Clone H57-597) | BioLegand | Cat# 127806, RRID:AB_1134188 | FC (1:200) |
| Antibody | Anti-IL-7RαBV650 (Rat monoclonal, Clone A7R34) | BioLegand | Cat# 135043, RRID:AB_2629681 | FC (1:200) |
| Antibody | Anti-PD-1 APC (Rat monoclonal, Clone RMP1-30) | BioLegand | Cat# 109112, RRID:AB_10612938 | FC (1:200) |
| Antibody | Anti- LPAM-1 (Integrin α4β7) PE (Rat monoclonal, Clone DATK32) | BioLegand | Cat# 120606, RRID:AB_493267 | FC (1:200) |
| Antibody | Biotin anti-mouse CD3 antibody | Biolegend | Cat# 100244 RRID:AB_2563947 |
60 μg/ml |
| Antibody | Biotin anti-mouse CD4 antibody | Biolegend | Cat# 100404 RRID:AB_312688 |
60 μg/ml |
| Peptide, recombinant protein | Streptavidin | SouthernBiotech | Cat# 7100–01 | 60 μg/ml |
| Sequence-based reagent | Cyrib_F | This paper | PCR primers | AGGAGCTGGCCACGAAATAC |
| Sequence-based reagent | Cyrib_R | This paper | PCR primers | GGCGTACTAGTCAAGGCTCC |
| Sequence-based reagent | Actb_F | This paper | PCR primers | CCTGAACCCTAAGGCCAACC |
| Sequence-based reagent | Actb_R | This paper | PCR primers | ATGGCGTGAGGGAGAGCATA |
| Commercial assay or kit | RNeasy Plus Micro Kit | QIAGEN | Cat# 74034 | |
| Commercial assay or kit | SuperScript IV Firs-Strand Synthesis Reaction, | Thermo Fisher Scientific | Cat# 18091050 | |
| Commercial assay or kit | PowerUp SYBR Green Master Mix | Thermo Fisher Scientific | Cat# A25741 | |
| Commercial assay or kit | Liver dissociation kit, mouse | MACS | Cat# 130-105-807 | |
| Chemical compound, drug | DTT | Fisher Scientific | Cat# BP172-5 | (1 mM) |
| Software, algorithm | Image Studio Lite | RRID:SCR_013715 | v5.2.5 | |
| Software, algorithm | FlowJo | FlowJo | RRID:SCR_008520 | v10.7.1 |
| Software, algorithm | GraphPad Prism | GraphPad Software | RRID:SCR_002798 | v9 |
| Other | Ghost Dye Violet 510 Viability dye | Tonbo Bioscience | Cat# 13–0870 T100 | FC (1:1000) |