| Model development in exploring sex-biased hybrid dysfunction |
How can models of classic Haldane’s rule be extended to provide predictions for sex-biased hybrid dysfunction in taxa lacking heteromorphic sex chromosomes? |
| What new models can be developed to explain sex-biased hybrid dysfunction in taxa with homomorphic GSD, ESD, haplodiploidy, and hermaphroditism? |
| Relative influence of factors causing Haldane’s rule |
What is the balance across sexual systems of forces that reinforce and oppose hybrid dysfunction biased toward a particular sex? |
| Which mechanisms most commonly explain exceptions to classic Haldane’s rule, and how do they link to phylogeny? |
| Associating sex-biased hybrid dysfunction with developmental timing over life history |
Under what circumstances do alternative mechanisms for sex-biased hybrid dysfunction affect predictions differently for sterility than for inviability? |
| Is sex-biased hybrid dysfunction truly more prevalent for sterility than for inviability, and if so, why? |
| To what extent does sex bias in pre-zygotic reproductive isolation associate with sex bias in post-zygotic hybrid dysfunction? |
| Connecting to other features of reproductive isolation |
How do predictions for sex-biased hybrid dysfunction intersect with parent-of-origin asymmetries (Darwin’s corollary to Haldane’s rule)? |
| What is the extent and biological significance of within-species genetic variation for sex-biased hybrid dysfunction? |
| Approaches and methodologies to investigate sex-biased hybrid dysfunction |
What is the relative contribution of divergence in regulatory and coding sequence to sex-biased hybrid dysfunction, and how do they conform to alternate theoretical expectations? |
| How might inference of different rates of accumulation of sex-biased hybrid dysfunction from ‘speciation clock’ analysis inform alternate explanatory models? |
| How might taxa with multiple sex chromosomes, neo-sex chromosomes, and autosomal paleo-sex chromosomes be exploited to inform alternate models for sex-biased hybrid dysfunction? |
| How can analysis of hybrid zones and hybrid populations differentiate among alternative explanations for sex-biased hybrid dysfunction? |
| What do experimental manipulations of sex using hormone treatments, transgenic alterations, and karyotype perturbations indicate for general causes of sex-biased hybrid dysfunction? |
| How can alternative causes of sex-biased hybrid dysfunction be distinguished by testing for differences in the ontogenetic timing at which dysfunctional development manifests? |
