Extended Data Fig. 4. Rragc-mutant ‘reverse’ BM chimeras exhibit shortened longevity.
a. Percentage of the indicated cell populations in the spleen of 18-month-old BM-Rragc+/+ (n = 9) and BM-RragcS74C/+ (n = 10) male mice. (B: B cells, T: T cells, Neu: neutrophils, Mon: monocytes, NK: natural killers, DC: dendritic cells, Mac: macrophages and Eos: eosinophils. b. Hematological parameters (hemoglobin and hematocrit) measured in 18-month-old BM-Rragc+/+ (n = 10) and BM-RragcS74C/+ (n = 11) male mice. c. qRT–PCR analysis of genes of the senescence-associated secretory phenotype (SASP) genes in kidneys from 18-month-old BM-Rragc+/+ (n = 12) and BM-RragcS74C/+ (n = 12) male mice. Data are presented as mean values ± SD. d. qRT–PCR analysis of genes of the senescence-associated secretory phenotype (SASP) in pancreas from 18-month-old BM-Rragc+/+ (n = 12) and BM-RragcS74C/+ (n = 12) male mice. Data are presented as mean values ± SD. e. Incidence of infiltrated inflammatory cells in the indicated tissues from 400 days-old Rragc+/+ (n = 5) and RragcS74N/+(n = 5) male hosts. f. Quantification of SA-β-gal+ area within the kidney area (left), p21 in kidney (center) and liver (right) from 400 days-old Rragc+/+ (n = 5) and RragcS74N/+(n = 5) male hosts. Data are presented as mean values ± SD. g. Quantification of inflammatory cytokines in sera from 390 days-old Rragc+/+ (n = 9) and RragcS74N/+(n = 7) male hosts measured by Legendplex assay using flow cytometry. Statistical significance in a, b and f was calculated by two-tailed Student’s t-test. Statistical significance in c and d was calculated by two-way ANOVA. Statistical significance in e and g was calculated by two-sided Fisher exact test.