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. 2024 Jun 7;4(8):1102–1120. doi: 10.1038/s43587-024-00635-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a, Quantification of neutrophils in peripheral blood before and after a 5-day treatment with anti-Gr1 or isotype control antibody from isotype-treated Rragc^+/+ (n = 4), anti-Gr1-treated Rragc^+/+ (n = 4), isotype-treated Rragc^S74N/+ (n = 4) and anti-Gr1-treated Rragc^S74N/+ (n = 3) 24-mo-old male mice. b, WBC count in isotype-treated Rragc^+/+ (n = 5) and Rragc^S74N/+ (n = 7), anti-Gr1-treated Rragc^+/+ (n = 6) and Rragc^S74N/+ (n = 7) 24-mo-old male mice. c, Quantification of cytokines in sera from isotype-treated Rragc^S74N/+ (n = 4) and anti-Gr1-treated Rragc^S74N/+ (n = 4) 24-mo-old male mice in a 5-day treatment measured by Legendplex assay. d, qRT–PCR analysis of SASP genes in the kidneys from isotype-treated Rragc^+/+ (n = 5) and Rragc^S74N/+ (n = 7), anti-Gr1-treated Rragc^+/+ (n = 6) and Rragc^S74N/+ (n = 7) 18-mo-old male mice. e, Quantification of SA-β-gal⁺ area within the kidney area of isotype-treated Rragc^+/+ (n = 5) and Rragc^S74N/+ (n = 7), anti-Gr1-treated Rragc^+/+ (n = 6) and Rragc^S74N/+ (n = 7) of 18-mo-old mice. f, Dermal thickness in back skin of the same mice as in e. g, Quantification of IHC of CD45⁺ cells in skin from the same mice as in f. h, Incidence of infiltrated inflammatory cells in livers from mice in e. i, Kaplan–Meier survival curves of control (n = 5) and anti-Gr1-treated (n = 9) Rragc^+/+ mice (left) and controls (n = 6) and anti-Gr1-treated (n = 10) Rragc^S74N/+ mice (right). j, Survival since the first dose of anti-Gr1 of the mice in k. k, qRT–PCR analysis of Vcam1 in kidneys of young (4-mo-old) and old (18-mo-old) Rragc^+/+ (young, n = 8; old, n = 8) and Rragc^S74N/+ (young, n = 8; old, n = 8) male mice. l, Representative IHC staining of Vcam1 in kidney from 18-mo-old Rragc^+/+ and Rragc^S74N/+ mice. m, Quantification of transferred wt CD45.1.1 neutrophils by IF in kidneys from CD45.2.2 host males treated with PBS (Rragc^+/+ (n = 3); Rragc^S74mut/+ (n = 3)) or anti-Vcam1 (Rragc^+/+ (n = 3); Rragc^S74mut/+ (n = 3)). Statistical significance was assessed by one-tailed Student’s t-test (a); two-tailed Student’s t-test (e–g, j,k); two-sided Fisher’s exact test (h); two-way ANOVA (b–d,m); and log-rank test (l). Data (b,e–g,j,k,m) are mean ± s.d.

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