Table 1.
Summary of chronic unpredictable stress protocol.
| Day | Time | Stressful Event |
|---|---|---|
| 1 | AM | 2 hours - Tilted cage 45° |
| 1 | PM | Overnight - Food and water deprivation |
| 2 | AM | 4 hours - Wet bedding |
| 2 | PM | 1.5 hours - Restraint stress |
| 3 | AM | 3 hours - Light deprivation |
| 3 | PM | 2 hours - Tilted cage 45° |
| 4 | AM | 10 min - 5 Foot shocks 0.6 mA |
| 4 | PM | Overnight - Food and water deprivation |
| 5 | AM | 2 hours - Restraint stress |
| 5 | PM | 10 min - 5 Foot shocks 0.7 mA |
| 6 | AM | 4 hours - Wet bedding |
| 7 | AM | 2 hours - Tilted cage 45° |
| 7 | PM | 3 hours - Light deprivation |
| 8 | AM | 10 min - 5 Foot shocks 0.7 mA |
| 8 | PM | 4 hours - Wet bedding |
| 9 | AM | 3 hours - Light deprivation |
| 9 | PM | Overnight - Food and water deprivation |
| 10 | AM | 2 hours - Restraint stress |
| 10 | PM | 2 hours - Tilted cage 45° |
| 11 | AM | 4 hours - Wet bedding |
| 11 | PM | 3 hours - Light deprivation |
| 12 | AM | 2 hours - Tilted cage 45° |
| 12 | PM | 10 min - 5 Foot shocks 0.8 mA |
| 13 | AM | 2.5 hours - Restraint stress |
| 13 | PM | 3 hours - Light deprivation |
| 14 | AM | 4 hours - Wet bedding |
| 14 | PM | Overnight - Food and water deprivation |