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. 2024 Aug 5;19(8):1820–1835. doi: 10.1021/acschembio.4c00389

Figure 3.

Figure 3

Size-dependent heparin-induced dimerization of Nrp1. SEC-MALS chromatogram of Nrp1 a1a2b1b2 and A, heparin hexasaccharide (dp6); B, heparin octasaccharide (dp8); C, heparin decasaccharide (dp10); D, heparin dodecasaccharide (dp12); E, heparin tetradecasaccharide (dp14); F, heparin hexadecasaccharide (dp16); G, heparin octadecasaccharide (dp18); and H, heparin icosasaccharide (dp20). SEC-MALS chromatogram of I, R513A + heparin octadecasaccharide (dp18); J, K514A + heparin octadecasaccharide (dp18); K, R513A,K514A + heparin octadecasaccharide (dp18); L, R513A + heparin icosasaccharide (dp20); M, K514A + heparin icosasaccharide (dp20); N, R513A,K514A + heparin icosasaccharide (dp20); and O, a mix of heparin oligosaccharides (dp20, dp14, dp8). Gray lines are protein only, and black lines are protein incubated with oligosaccharide.