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. 2024 Aug 5;19(8):1820–1835. doi: 10.1021/acschembio.4c00389

Figure 5.

Figure 5

Neuropilin-1 binding to cell surface heparan sulfate. A, CHO-K1 cells titrated with recombinant wild-type Nrp1 a1a2b1b2 protein. B, Binding of wild-type Nrp1 a1a2b1b2 and mutants (1 μM) to CHO-K1 cells with and without treatment with heparin lyases I, II, and III (HSase). Statistics denote a one-way ANOVA with Tukey’s multiple comparison test. ** p < 0.002 and **** p < 0.0001. C, HUVECs were titrated with recombinant wild-type Nrp1 a1a2b1b2 protein. D, Binding of wild-type Nrp1 a1a2b1b2 and mutant (3 μM) to HUVECs with and without HSase treatment. Specific binding (total binding–nonspecific binding) is shown for each condition in this figure. Statistics denote a two-way ANOVA with Tukey’s multiple comparison test. * p < 0.033 and ** p < 0.002.