Figure 6.

Alteration of neuropilin-1 and heparan sulfate binding impairs subcutaneous B16 tumor growth. A, Schematic of subcutaneous B16–F10 tumor model. B, Tumor growth curves of bilateral subcutaneous B16–F10 tumors in wild-type and mutant Nrp1 mice. Significance between Nrp1^+/+ and Nrp1^D/–. C, Tumor growth curves of unilateral subcutaneous B16–F10 tumors in wild-type and mutant Nrp1 mice. Significance between Nrp1^D/D and Nrp1^D/–. There is some variability between the bilateral and unilateral models, as observed from differences in the growth curves of the Nrp1^D/D mice. Statistics denote a two-way ANOVA with the Geisser–Greenhouse correction followed by a Fisher’s LSD test for each set of data. * p < 0.05 and ** p < 0.01.