Table 3. Determined Molar Masses (MMs) for Nrp1 a1a2b1b2:dp18 and Nrp1 a1a2b1b2:dp20 complexes.
solo |
+dp18 (5.2 kDa) |
+dp20 (5.8 kDa) |
expected
size + dp18 |
expected
size + dp20 |
||||||
---|---|---|---|---|---|---|---|---|---|---|
MM (kDa) | error (%) | MM (kDa) | error (%) | MM (kDa) | error (%) | 1:1 complex | 2:1 complex | 1:1 complex | 2:1 complex | |
Nrp1 | 80 | 5 | 140 | 7 | 183 | 3 | 85 | 165 | 86 | 166 |
R513A | 79 | 1 | 80 | 3 | 83 | 3 | 84 | 163 | 85 | 163 |
K514A | 79 | 2 | 92 | 2 | 97 | 4 | 84 | 163 | 85 | 163 |
R513A, K514A | 78 | 2 | 79 | 1 | 77 | 1 | 83 | 161 | 84 | 161 |