Figure 3. Immunoblots (A) and their quantification (B) for glutamic acid decarboxylase (GAD).

The amounts of GAD in the pancreas lysates were determined by immunoblotting using the anti-GAD 65/67 antibody. The protein bands on the immunoblots were analysed by ImageJ software and the results were expressed as the relative intensity in the y-axis; the STZ-treated sample was set to one (1)

*P < 0.05 indicates a comparison with values of normal rats

Control, STZ, STZ/IN-1, and STZ/IN-2 represent the results for the immunoblot with pancreas lysates of the normal, STZ-diabetic, and STZ/albumin (IN-1 and IN-2)-treated rats, respectively

GAPDH = glyceraldehyde 3-phosphase dehydrogenase; STZ = streptozotocin