Figure 1.

Novel bioPROTACs potently degrade cytosolic proteins in T cells. (A) Top: Cartoon diagramming abbreviated endogenous proteasomal degradation. Bottom: Cartoon depicting proposed bioPROTAC design and implementation. (B) Top: Cartoon depicting archetype of lentiviral payload used to screen degrons for bioPROTAC construction in Jurkat cells. Bottom: Comparison of degron efficacies. Fluorescence was measured by flow cytometry. Dots represent biological replicates, and error bars show SEM. (C and D) Left: Cartoons describing lentiviral payloads used to test bioPROTAC efficacy in Jurkat cells. Middle: Flow cytometry histograms of the diagrammed lentiviral payloads in the left panel alongside controls. Histograms are representative of three independent experiments. Right: Quantification of flow cytometry histograms. Relative GFP fluorescence was calculated by normalizing measured median GFP fluorescence by the median fluorescence of the reporter only control. Each dot represents a technical replicate, and error bars show SEM. Data is representative of three independent experiments. An unpaired t test was used for all statistical comparisons. *P < 0.01.