| piGEM1 |
this study.
negative control.
encodes for sfGFP and norR, does
not contain any promoter |
| piGEM3 |
this study. encodes for sfGFP and norR under the control of the already characterized inducible
promoter pNorV |
| piGEM2.1 |
this
study. encodes for sfGFP and norR under the control of the inducible promoter pNorVβ.
this construct contains 1 RBS directly
upstream of sfGFP
|
| piGEM2.2 |
this study. encodes for sfGFP and norR under the control of the inducible promoter pNorVβ.
this construct contains 2 RBS and a
spacer upstream of sfGFP
|
| piGEM2.3 |
this study. encodes for sfGFP and norR under the control of the inducible promoter
pNorVβ.
this construct contains 3 RBS and a
spacer upstream of sfGFP
|
| piGEM2.2N |
this study. encodes for sfGFP under the control
of the inducible promoter pNorVβ. this construct contains 2 RBS and a spacer upstream of sfGFP. this construct does not contain norR
|
| pSBinit |
retrieved from ref (58), addgene #110100. . coli entry and expression vector for FX cloning
system, N-terminal pelB signal sequence
and C-terminal myc and 6× HisTag |
| purNb1 |
this study. nanobody candidate
VHH#2B cloned into pSBinit expression
vector via FX cloning |
| purNb2 |
this study. nanobody candidate VHH#3E cloned into pSBinit expression
vector via FX cloning |
| purNb3 |
this study. nanobody candidate VHH#12B cloned into pSBinit
expression vector via FX cloning |
| purNb4 |
this study. nanobody candidate VHH#2B–VHH#2B cloned into pSBinit expression vector via FX cloning |
| purNb5 |
this study. Nanobody candidate VHH#3E–VHH#3E cloned into pSBinit expression
vector via FX cloning |
| purNb6 |
this study. nanobody candidate VHH#12B–VHH#12B cloned into pSBinit expression vector via FX cloning |
| purNb7 |
this study. nanobody candidate VHH#2B–VHH#3E cloned into pSBinit expression
vector via FX cloning |
| purNb8 |
this study. nanobody candidate VHH#2B–VHH#12B cloned into pSBinit expression vector via FX cloning |
| purNb9 |
this study. nanobody candidate VHH#3E–VHH#12B cloned into pSBinit expression
vector via FX cloning |
| pSS |
this
study. plasmid encoding HlyB and HlyD required for the
HlyA secretion system under a constitutive promoter (J23100). Contains
chloramphenicol resistance gene |
| pNb |
this study. plasmid encoding HlyA and myc-tag under inducible pBad promoter with restriction sites allowing the
cloning of the different nanobodies in front of the two tags. Contains
ampicillin resistance gene |
| pNb1 |
this study. nanobody candidate VHH#2B cloned into pNb plasmid
via FX cloning |
| pNb2 |
this study.
nanobody candidate VHH#3E cloned into pNb plasmid
via FX cloning |
| pNb3 |
this study.
nanobody candidate VHH#12B cloned into pNb plasmid
via FX cloning |
| pNb5 |
this study.
nanobody candidate VHH#3E–VHH#3E cloned into pNb plasmid via FX cloning |
| pNb7 |
this study. nanobody candidate VHH#2B–VHH#3E cloned into pNb plasmid via
FX cloning |
| pNb8 |
this study.
nanobody candidate VHH#2B–VHH#12B cloned into pSBinit expression vector via FX cloning |
| pNO1_Nb1 |
this study. nanobody candidate VHH#2B cloned into piGEM2.1
plasmid via Gibson |
| pNO3_Nb1 |
this study. nanobody candidate VHH#2B cloned into
piGEM2.3
plasmid via Gibson |
