Figure 3.
TPHi Attenuates Muscularization of Small Pulmonary Arteries and Cardiac Fibrosis in the SuHx Rat Model
(A to C) Representative images of small peripheral pulmonary arteries in hematoxylin and eosin (H&E), Masson’s Trichrome, and α-smooth muscle actin (SMA) staining, respectively. Scale bars: 25 μm. Bar graphs: (B) percentage of perivascular collagen. n = 20-40 vessels per animal; n = 7-8 animals. (C): Peripheral pulmonary artery muscularization. n = 20-40 vessels per animal; n = 7-8 animals. (D) Masson’s Trichrome staining of interstitial collagen in RV tissue. n = 4-7 individual animals. Mean ± SEM, ANOVA–Bonferroni post hoc test. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.