Figure 4.

Immunofluorescent Evaluation of Key Targets in the Rat Lung

(A) Representative photographs showing proliferating cell nuclear antigen (PCNA) staining. PCNA signal in red, 6-diamino-2-phenylindole (DAPI) in blue, and red blood cells in yellow, quantification to the right. (B) Representative photographs showing accumulation of CD3⁺ T cells in the lungs of SuHx animals, which is not present in the treated group. CD3 signal in red, DAPI in blue, and red blood cells in yellow, quantification to the right. (C and D) Representative photographs showing accumulation of F4/80⁺ and CD68⁺ macrophages in the lung of SuHx animals, which is absent after the treatment. CD68 signal in red, DAPI in blue, quantification to the right. Mean ± SEM, ANOVA–Bonferroni post hoc test. ∗∗∗P < 0.001.