TABLE 3.
The mutations of amino acids except S proteins during the continuous passage of PDCoV CH/XJYN/2016 in LLC-PK cellsa
| ORF1a | ORF1b | E | M | N | NS7 | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Amino acid position | Nsp2 | Nsp3 | Nsp4 | Nsp5 | Nsp7 | Nsp12 | Nsp14 | |||||||||||||
| Passages | 131 | 828 | 983 | 1,297 | 1,549 | 1,589 | 2,200 | 2,682 | 3,134 | 3,165 | 116 | 228 | 673 | 1,872 | 14 | 73 | 157 | 329 | 94 | 161 |
| P0 | A | G | A | D | T | T | A | N | A | A | H | T | D | I | Y | I | P | E | L | G |
| P10 | A | G | A | D | T | A | V | N | E | S | Y | T | Y | I | Y | I | P | E | P | G |
| P20 | A | G | A | D | M | A | V | N | E | S | Y | T | Y | I | Y | I | P | E | P | E |
| P30 | A | G | A | D | M | A | V | N | E | S | Y | T | Y | I | Y | I | S | E | P | E |
| P40 | A | G | A | D | M | A | V | N | E | S | Y | T | Y | I | C | I | S | E | P | E |
| P50 | A | G | A | D | M | A | V | N | E | S | Y | T | Y | I | C | I | S | E | P | E |
| P60 | A | G | A | D | M | A | V | N | E | S | Y | T | Y | I | C | I | S | E | P | E |
| P70 | A | G | A | D | M | A | V | N | E | S | Y | T | Y | I | C | I | S | E | P | E |
| P80 | A | G | A | D | M | A | V | N | E | S | Y | S | Y | I | C | I | S | E | P | E |
| P90 | A | G | A | D | M | A | V | N | E | S | Y | S | Y | I | C | I | S | E | P | E |
| P100 | A | G | A | D | M | A | V | N | E | S | Y | S | Y | I | C | I | S | E | P | E |
| P110 | A | G | A | D | M | A | V | N | E | S | Y | S | Y | I | C | I | S | E | P | E |
| P120 | S | G | A | D | M | A | V | N | E | S | Y | S | Y | I | C | I | S | E | P | E |
| P130 | S | G | A | D | M | A | V | N | E | S | Y | S | Y | T | C | S | S | E | P | E |
| P140–P210 | S | G | E | D | M | A | V | N | E | S | Y | S | Y | T | C | S | S | E | P | E |
| P220 | S | G | E | E | M | A | V | K | E | S | Y | S | Y | T | C | S | P | E | P | E |
| P230 | S | G | E | E | M | A | V | K | E | S | Y | S | Y | T | C | S | P | D | P | E |
| P240 | S | W | E | E | M | A | V | K | E | S | Y | S | Y | T | C | S | P | D | P | E |
a
There are mutations detected in Nsp2, Nsp3, Nsp4, Nsp5, Nsp7, Nsp12, Nsp14, E, M, N, and NS7, but not from Nsp6, Nsp8, Nsp9, Nsp10, Nsp11, Nsp13, Nsp15, Nsp16, and NS6 proteins.
b
Grey indicates that the amino acid at this site has mutated. Dark gray indicates that the amino acid at this site mutated for the second time in different generations.