Fig 3.

Dabrafenib, but not Regorafenib, reduces viral dsRNA levels. A549 cells were infected with ZIKV (MOI = 3 PFU/cell) and subsequently treated with DMSO (vehicle control), Dabrafenib (10 µM), or Regorafenib (2.5 µM). ZIKV infection was visualized at 24, 48, and 72 hpi by immunofluorescence staining for ZIKV E protein (red) and dsRNA (green). Nuclei were stained with Hoechst 33342 (blue). Scale bars, 100 µm. Dot plot shows image-based quantification of the integrated density of dsRNA in individual cells. Data are expressed as mean ± SEM. Data from one of three independent experiments with similar results are shown. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (unpaired Student’s t test vs “DMSO”).