Extended Data Fig. 4 |. Differences in azaserine degradation in various media.

a, Azaserine titer continues to decrease after removal of S. albus cells. The azaserine-producing S. albus was grown in TSB medium. After 24 h, the cells were removed from culture broth using a 0.22-μm sterile filter, the filtrate was incubated at 30 ℃ (labeled as 0 h), and the azaserine concentration was monitored at different time points. b, Azaserine is stable in fresh TSB medium of normal pH 7.3 or adjusted pH 8.4. Equal volume of azaserine stock was added to a final concentration of about 35 mg/L at 0 h. Data are mean ± s.d.; n = 3 biological (a) or technical (b) replicates.