Extended Data Fig. 5 |. Purified P450-T2 WT and mutant catalyzing the reaction of styrene with azaserine in vitro.

a, Coomassie Blue stained SDS-PAGE gel of purified protein P450-T2 WT (left) and P450-T2–5 mutant (right). Each lane is a sample from fractions collected during ion exchange purification. b, Purified P450-T2 WT and P450-T2–5 mutant proteins catalyze the reaction in vitro with high diastereoselectivity. 5th, P450-T2–5 mutant. Reaction conditions: 5 mM styrene, 5 mM azaserine, 10 μM enzyme, 10 mM Na₂S₂O₄, 5 vol% ethanol, M9-N buffer, conducted at 22 °C under aerobic condition for 18 h. P_total, sum area for all diastereomers. Grey bars indicate the Dr. Data are mean ± s.d.; n = 3 reaction replicates.