Eyes were injected with NMDA ± DZN (SAHH inhibitor) at P6, vehicle or DZN at P7, and eyes harvested at 4 hrs after the last injection. Retinal sections were for cell death (TUNEL) or antibodies to cFos and Sox2, pS6 and pStat3 (a). The calibration bars in a represent 50 μm. (b-f) Histograms represent the mean (bar±SD) and each dot represents one biological replicate (blue lines connect control and treated eyes from the same individual) for levels of immunofluorescence. Significance of difference (p-value) was determined using a paired t-test. Abbreviations: ONL – outer nuclear layer, INL – inner nuclear layer, IPL – inner plexiform layer, GCL – ganglion cell layer, ns – not significant.