Extended Data Fig. 6: General morphology analysis of T.A and hearts from AAV6-treated cohort.
a) Heart sections of control mice or mdx4cv males treated with single, dual, or triple AAV6 vectors stained with H&E or double-immunolabeled for dystrophin and laminin (Scale bars: 100μm). Dashed rectangles represent a higher magnification view. b) Percentage of dystrophin-positive myofibers, c) fiber area, d) minimal fiber diameter measured using T.A cross sections (n=4, more than 1000 fiber per each sample). For b, data represent mean ± s.e.m. In c and d, the average values are shown on top of the violin bars. The solid line represents the median, while the dashed lines show the quartiles. μDys: micro-dystrophin. mDys: midi-dystrophin. fDys: Full-length dystrophin. Dys+: Dystrophin positive. vg: viral genome. kg: Kilogram