Fig. 7.

INF2 R218Q requires integrity of its actin polymerization activity to produce nuclear abnormalities. A Dynamics of wt INF2 during mitosis in MDCK cells expressing GFP-tubulin. Cells were stained with SiR-DNA. B Images of INF2 KO MDCK cells expressing INF2 R218Q and R218Q IA. (C) Box plots showing the intensity of the perinuclear F-actin and Cherry corresponding to INF2-1 R218Q and INF2-1 R218Q IA MDCK KO cells. More than 150 cells were examined; three independent experiments. D Percentage of MDCK KO cells expressing INF2-1 R218Q IA and INF2-2 R218Q IA exhibiting mild or severe nuclear phenotypes. More than 350 cells were examined; three independent experiments. E Images of an equatorial plane of INF2 KO MDCK cells expressing INF2 A149D and INF2 3LA. F Percentage of cells expressing INF2 A149D and INF2 3LA displaying mild or severe nuclear phenotypes. More than 300 cells were examined across three independent experiments. The contrast in the Cherry channel was increased as indicated to identify the cells expressing mutant INF2. Nuclei were visualized with DAPI. Scale bars, 5 μm (A) and 10 μm (B, E). **, p < 0.01; ***, p < 0.001