Fig. 2.

Impact of equilibration duration on kidney organoids during slow freezing: a three-time-point study. (A) The comparison of sizes was conducted among all slow-freezing conditions in contrast to the control. Despite utilizing the same freezing method across all conditions, the variable factor was the duration of equilibration. Following freezing, a decrease in size was observed across all subtypes of slow freezing, succeeded by a subsequent normal growth pattern. The kidney organoids utilized in this study were of the BJFF type, and their age at Day 0 was 35 days. (B) An elucidation of the three experimental conditions within this segment of the study is shown. (C) Immunocytochemistry with sample clearing was employed to examine the features of proximal tubule (LTL), distal tubule (CDH1), and podocyte (PODXL) in all conditions on day 42. The scale bar: 100 μm