Fig. 8.

Illustration of the experiment testing the vitrification method on organoids of different ages. Additional experiment testing 5 μM cisplatin toxicity on organoids at 14, 21, and 35 days to demonstrate the effectiveness of vitrification across different ages. After thawing, organoids were cultured until day 49 of differentiation and exposed to cisplatin for 24 h. Samples were harvested on day 56 of differentiation for immunohistochemical assessment (LTL: red, CDH1: green, PODXL: white). For the day 35 kidney organoids, they were thawed from our stocks after 7 weeks to check the effect of storage in the − 150 °C freezer. Scale bars: 80 μm