Fig. 6.

Augmented DCs activation and Th2 expansion in Cc10^−/− mice post-HDM challenge. A Flow cytometry of lung CD11b⁺CD103⁻ and CD11b⁻CD103⁺ DCs (gated on live CD45⁺CD11c⁺HLA-DR⁺ cells). B, C Quantification of DC subsets (n = 6/group). D–F qRT-PCR analysis of Il6, Tnfa, Il12 mRNA levels in lung DCs post-HDM exposure (n = 3/group). G FITC-dextran uptake by lung DCs measured via flow cytometry. Data are representative for three independent experiments (n = 4/group). H Flow cytometry of Th2 (CD4⁺IL-4⁺) and Th17 (CD4⁺IL-17A⁺) cells in lungs. I, J Quantification of Th cell subsets (n = 6/group). Data are mean ± SEM, representing three experiments. P values from Student’s t-test (G) and one-way ANOVA with Games-Howell test. *P < 0.05, **P < 0.01, ns not significant