Fig. 7.

CC10 modulates immunological activity of BMDCs In Vitro. A–C Flow cytometric analysis of CD86, CD40, HLA-DR on BMDCs post CC10 or dexamethasone (Dex) and LPS (200 ng/mL) treatment. The graph shows averages from 4 independent experiments. D FITC-dextran endocytosis by immature BMDCs. Data from 3 independent experiments. E–I ELISA for cytokines in supernatants of LPS-stimulated BMDCs pre-treated with CC10 or Dex (n = 3/group). J Western blot analysis of IκBα and p65 phosphorylation in LPS-stimulated BMDCs with or without CC10. β-actin used as loading control. Data from two experiments with similar results. All data are shown as mean ± SEM. P values from one-way ANOVA with Games-Howell test. *P < 0.05, **P < 0.01, ns not significant