Fig. 2.

AP-1γ1 silencing affects ORF2i subcellular localization and colocalization with various viral/host cell markers. (a-d) At 6 d.p.e., PLC3/HEV cells were transfected with siRNA targeting AP-1γ1 (siAP-1γ1), with a non-targeting control siRNA (siCTL). At 3 d.p.t, cells were permeabilized with cold methanol and 0.5% Triton X-100 and double-stained with the indicated antibodies. For each double staining, MOC of the ORF2i labelling in the cellular marker/ORF3 labelling and MOC of the STX6 labelling in the TGN46 labelling were determined using the whole cell as ROI. Each data dot in the bar chart represents a cell field, and the total number of ORF2-positive cells used to calculate the MOC is indicated. Displayed results come from biological replicates. Mann-Whitney test, ****p < 0.0001