Fig. 4.

AP-1 complex pharmacological inhibition by A5 impairs ORF2i subcellular localization and colocalization with various viral/host cell markers. (a-g) At 6 d.p.e., PLC3/HEV cells were treated with H₂O or A5 (150µM) and fixed after 3 days of treatment. Then, cells were permeabilized with cold methanol and 0.5% Triton X-100 and double-stained with the indicated antibodies. For each double staining, MOC of the ORF2i labelling in the cellular marker/ORF3 labelling was determined using the whole cell as ROI. Each data dot in the bar chart represents a cell field, and the total number of ORF2-positive cells used to calculate the MOC is indicated. Displayed results come from biological replicates. Mann-Whitney test, ****p < 0.0001