Fig. 4.

Identification of potential specific CDKL5 phosphotargets in CDKL5 human neurons. A Label free quantitative phospho-MS (LFQPMS) of phosphorylated CDKL5 neuronal proteins (left = higher in wild-type), highlighting sites containing the CDKL5 consensus motif. Volcano plot shows significantly changed proteins from Perseus analysis (BH FDR < 0.05, S0 = 0.1; n = 6 CDKL5 isogenic control neurons, n = 3 CDKL5 p.(Arg59*) neurons). B Conservation of phosphorylated sites in proteins containing the CDKL5 motif. C Phosphorylation level of targets in each sample were consistent between replicate samples. Each coloured line represents phosphorylation levels of the four targets identified in volcano plot in Fig. 4A (PPP1R35 in green, GTF2I in blue, GATAD2A in red and ZNF219 in purple)