Fig. 3.
The binding energies and surface electrostatic potentials of the TadA-DNA complexes. (A) The binding energy ratios of TadA to DNA substrate, using the absolute values of TadA-Cas9n as the unit of 1. Data are presented as the mean ± SD. (B) The energy terms of TadA with the DNA substrate using the absolute values of the VDW energy of TadA7.10:TadA7.10 as the unit of 1. Data are presented as mean ± SD. (C) The surface electrostatic potentials of TadA calculated by APBS, on a scale from − 3 to 3 kBT/ec. Red represents negative electrostatic potential, blue represents positive electrostatic potential, and white is neutral