FIGURE 1.

Nanoparticle tracking analysis (NTA) and electron micrographs of exosomes (exo) isolated from short-term endometriotic tisesue cultures and serum from endometriosis patients and healthy controls. (A and B) Size distribution of representative endometriotic tissue exosomes and serum samples (A) and mean endometriotic tissue exosome size (n = 5) (B) compared with the size of endometriosis serum exosomes (n = 7). (C) Representative micrographs of negatively stained endometriotic tissue exosomes showing size, purity, and the typical cup shape, and immunoelectron microscopy with mAbs and silver-enhanced immunogold staining of the tetraspanins CD63 and CD81; and CA125, often used as an ovarian cancer marker. (D) Representative experiment of NTA analysis and negative contrast staining of exosomes from serum of healthy controls.