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. 2024 Jul 10;213(5):567–576. doi: 10.4049/jimmunol.2300781

FIGURE 4.

FIGURE 4.

Endometriotic tissue–derived exosomes (exo) carry functional FasL and TRAIL that trigger apoptosis of Jurkat cells in vitro. (A) Immunoelectron microscopy of endometriotic tissue exosomes stained with mAbs against the proapoptotic ligands FasL and TRAIL. (B) Immunoflow cytometry of endometriotic tissue exosomes captured on latex beads coated with mAbs against FasL and TRAIL. The black curves represent negative isotype-matched mAb controls. (C) Average expression of FasL and TRAIL, measured by MFI normalized to isotype-matched mAb control (= 1) on endometriotic tissue exosomes compared with endometriosis serum exosomes and exosomes from healthy donors (n = 4). (D and E) Apoptosis of Jurkat cells induced by endometriotic tissue–derived exosomes (D) and by endometriosis serum exosomes (E) (n = 4). (F and G) Apoptosis of activated PBMCs induced by endometriotic tissue–derived exosomes (F) and by endometriosis serum exosomes (G) (n = 3).