Figure 2.

Treatment of S. aureus preformed biofilms with different combinations of bacteriophage phiIPLA-RODI and depolymerase Dpo7. 24-h biofilms formed by S. aureus 15981 (A), V329 (B), Newman (C), JE2 (D) and IPLA16 (E) were treated with 10⁸ or 10⁹ PFU/ml of phage (orange and green bars, respectively), 1 or 2 μM of depolymerase, or a combination of both antimicrobials. Biofilms were treated for 24 h at 37°C. TSB medium alone was added to the control wells (gray bars). The graphs show the viable cell counts observed after each treatment. Data represent the means ± standard deviations of three independent experiments. Bars with an asterisk are statistically different from the untreated control. *p-value <0.05; **p-value <0.01; ***p-value <0.001; and ****p-value <0.0001.