Figure 3.
Impact of the treatment with different combinations of bacteriophage phiIPLA-RODI and depolymerase Dpo7 on S. aureus preformed biofilms. 24-h biofilms formed by S. aureus 15981 (A) and V329 (B) were treated with 108 or 109 PFU/ml of phage (orange and green bars, respectively), 1 or 2 μM of depolymerase Dpo7, or a combination of both antimicrobials for 24 h at 37°C. TSB medium alone was added to the control wells (gray bars). The graphs show the amount of total biomass as determined by crystal violet staining and subsequent measurement of absorbance at 595 nm. Data represent the means ± standard deviations of three independent experiments. Bars with an asterisk are statistically different from the untreated control. *p-value <0.05; **p-value <0.01; ***p-value <0.001; and ****p-value <0.0001.