Figure 4.
Treatment of biofilms formed by S. aureus V329 with phiIPLA-RODI (109 PFU/ml), Dpo7 (2 μM), a combination of both or TSB alone (control). Biofilms were grown for 24 h at 37°C and subsequently treated for the same duration. After removal of the treatment, the attached cells were stained with SYTO® 9, propidium iodide and Wheat Germ Agglutinin (WGA) Alexa Fluor® 647 conjugate. Green represents live cells, red represents dead cells or eDNA and blue indicates the presence of polysaccharides with N-acetylglucosamine residues.