Fig. 1.

Shifting female flies to diet with different caloric content effects lifespan and mortality. A Schematic diagram of the experimental design: a group of female flies were aged on a low-calorie (L), or a high-calorie diet (H). Additional three groups of flies were shifted from H to L diet at day 20 (HLD20), 50 (HLD50), or 60 (HLD60), and another three from L to H diet on day 20 (LHD20), 50 (LHD50), or 60 (LHD60). B–I Survivorships (B, C) and mortality rates (D–I) of female flies shifted from H to L at day 20 (HLD20), day 50 (HDL50), or day 60 (HLD60) (B, D–F) or from L to H diet on day 20 (LHD20), day 50 (LHD50), or day 60 (LHD60) (C, G–I). Number of flies: L = 439, H = 402, HLD20 = 207, HLD50 = 217, HLD60 = 211, LHD20 = 207, LHD50 = 226, LHD60 = 218. Survivorship curves and mortality rate were analyzed by long-rank test JMP16 program