Fig. 2.

Shifting diets at different times during aging has immediate effects on female lifespan and mortality. Survivorships (A, B) and mortality rates (C–L) of female flies shifted from a high (H)- to a low (L)-calorie diet at day 10 (HLD10), day 20 (HLD20), day 30 (HLD30), day 40 (HLD40) or day 50 (HDL50) (C–G) or from L to H diet on day 10 (LHD10), day 20 (LHD20), day 30 (LHD30), day 40 (LHD40) or day 50 (LHD50) (H–L). Number of flies: L = 194, H = 227, HLD10 = 219, HLD20 = 225, HLD30 = 211, HLD40 = 222, HLD50 = 229, LHD10 = 231, LHD20 = 242, LHD30 = 199, LHD40 = 228, LHD50 = 224. Survivorships curves and mortality rate were analyzed by log-rank test JMP16 program