Fig. 4.

Effects of diet shifting on metabolism are age-dependent. Levels of glucose (A), trehalose (B), glycogen (C), triglycerides (D), protein (E), and weight (F) in female flies on days 20 and 50 before shifting, and 1, 2, and 5 days after shifting from a high- to a low-calorie diet (HL) or L to H diet (LH). Weight is an average weight per fly, calculated from the three biological replicates (10 flies per replicate). Error bars represent SEM. Data from 20 and 50 days were analyzed separately using a two-way ANOVA. Post hoc analysis was conducted using Tukey’s test, correcting for multiple comparisons. Results represent means ± SE of three biological replicates (10 flies per replicate). p: 0.05 (*), 0.01 (**), 0.001 (***), < 0.0001 (****)